The Franklin County Sheriff’s Office said they got a call around 5:20 Tuesday morning about 10-15 cows running loose on the interstate.
According to Sheriff Pat Melton, the driver of a trailer shipping 110 feeder cattle from Virginia to Kansas had fallen asleep while driving west near mile marker 53.
The Sheriff said the driver went off the road, flipped and crashed.
The driver and his passenger are both said to be doing well.
Roadways within a few miles of the crash were blocked off, although I-64 east bound and the right lane of I-64 west bound are currently open.
However, Melton said before the roads could be blocked, one cow was hit and killed near the crash scene.
He also told ABC 36 that three more cows were hit when two drivers cut across the grass to turn around illegally, killing two of the cattle.
The Sheriff reported that the third was on the ground and was struck a second time by a pickup truck. It has since been euthanized.
"The price of cattle is sky high," said Melton. "We want to save as many as we possibly can."